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Targeting Glioblastoma Cell Invasion Using Zebrafish Models for 4D Quantitative Imaging
Glioblastoma is an aggressive primary brain tumor with a 5-year survival rate of less than 5%. The ability of glioblastoma cells to invade surrounding brain tissue presents the primary challenge for the success of focal therapeutic approaches. In the Greenwood lab, our research focuses on glioblastoma cell invasion and mechanisms that slow or stop cancer cell movement. To study the invasion process, we inject fluorescently dyed human glioblastoma cells into the brain of embryonic zebrafish. The zebrafish are translucent at this stage allowing us to image into the brain and monitor glioblastoma cells in action. By altering the expression of proteins in the brain environment, we expect to identify specific proteins that simulate or inhibit glioblastoma cell invasion with potential to develop new approaches for cancer therapy.
|Single-cell imaging of human glioblastoma cell invasion in a zebrafish brain. Fluorescently dyed human glioblastoma cells 48hrs after transplantation into the brain of an embryonic zebrafish with eGFP expressing blood vessels. A) Brightfield image of zebrafish with location of confocal 3D imaging. B) Glioblastoma cells (red) can be seen invading into brain tissue from primary mass marked with a yellow arrow. White arrows indicate individual cells migrating along blood vessels (green).|